Table 1 List of PCR primer sequences
From: CD4+CCR8+ Tregs in ovarian cancer: a potential effector Tregs for immune regulation
Gene name | Forward primer(5′ → 3′) | Reverse primer(5′ → 3′) |
|---|---|---|
CCR1 | CAGCCTTCACTTTCCTCACG | AACGGACAGCTTTGGATTTCTT |
CCR2 | CCACATCTCGTTCTCGGTTTATC | CAGGGAGCACCGTAATCATAATC |
CCR3 | TCCTTCTCTCTTCCTATCAATC | GGCAATTTTCTGCATCTG |
CCR5 | CTTCTGGGCTCCCTACAACA | CAGATATTTCCTGCTCCCCA |
CCR6 | TTCAGCGATGTTTTCGACTCC | GCAATCGGTACAAATAGCCTGG |
CCR7 | TGAGGTCACGGACGATTACAT | GTAGGCCCACGAAACAAATGAT |
CCR8 | CAAGCCCCTGTGATGCGGAAC | AGACCACAAGGACCAGGATGACC |
CCR9 | ATGTCAGGCAGTTTGCGAG | TGCAGTACCAGTAGACAAGGAT |
CCR10 | TGCTGGATACTGCCGATCTACTG | TCTAGATTCGCAGCCCTAGTTGTC |
CXCR3 | CCGTCCAGTGGGTCTTTGG | AGGGCTCCTGCGTAGAAGTTG |
CXCR4 | TGTCATCTACACAGTCAACCTC | CAACATAGACCACCTTTTCAGC |
CXCR5 | TACCCGCTAACGCTGGAAATGGAC | CACGGCAAAGGGCAAGATGAAGAC |
CXCR6 | ATGCCATGACCAGCTTTCACT | ATGCCATGACCAGCTTTCACTG |
CCL1 | AATACCAGCTCCATCTGCTCCAA | GAACCCATCCAACTGTGTCCAAG |
CCL8 | TGGAGAGCTACACAAGAATCACC | TGGTCCAGATGCTTCATGGAA |
CCL16 | GCCCACTGAGAGGATGAAGG | TACTTCAGGCAGCAGTTGGG |
CCL18 | TGCCCAGCATCATGAAGG | TCAGGCATTCAGCTTCAGG |
β-actin | GAGCTACGAGCTGCCTGACG | GTAGTTTCGTGGATGCCACAG |