Fig. 5

Chemotactic experiment of CD4⁺CCR8⁺ Tregs and expression levels of CCR8 ligands in ovarian tumor and paracancerous tissues A Chemotaxis assay of CD4⁺CCR8⁺ Tregs in medium group, paracancerous tissue culture supernatant group and OC tissue culture supernatant group (original magnification 200 ×). B Gene expression levels of CCL1, CCL8, CCL16 and CCL18 in ovarian tumor (n = 32) and paracancerous tissues (n = 32). C The protein expression levels of CCL1 and CCL18 in ovarian tumor and paracancerous tissues. D Expression levels of CCL1 and CCL18 in ovarian tumor and paracancerous tissues (original magnification 400 ×). a Expression of CCL1 in paracancerous tissues; b Expression of CCL18 in paracancerous tissues; c–f The expression of CCL1 in OC tissue was 0 (−), 4 ( +), 8 (+ +), 12 (+ + +). g–h The expression of CCL18 in OC tissue was 0 (−), 4 ( +), 8 (+ +), 12 (+ + +). E The contents of CCL1 in the plasma of healthy individuals (n = 35), the plasma of OC patients (n = 36), the culture supernatants of ovarian tumor (n = 15) and paracancerous tissues (n = 35); the contents of CCL18 in the plasma of healthy individuals (n = 17), the plasma of OC patients (n = 18), the culture supernatants of ovarian tumor (n = 15) and paracancerous tissues (n = 20). Each experiment was performed triplicated. Data are presented as means ± SD and analysed with Student’s t-test. (*P < 0.05; **P < 0.01; ****P < 0.0001)