Fig. 3

Phenotypic characteristics of CD4⁺CCR8⁻/CCR8⁺ Tregs in HC-PBL group, OC-PBL group and OC-TIL group A Representative flow cytometry analysis of inhibitory phenotype molecules of CD4⁺CCR8⁻/CCR8⁺ Tregs in HC-PBL group. B Representative flow cytometry analysis of inhibitory phenotype molecules of CD4⁺CCR8⁻/CCR8⁺ Tregs in OC-PBL group. C Representative flow cytometry analysis of inhibitory phenotype molecules of CD4⁺CCR8⁻/CCR8⁺ Tregs in OC-TIL group. D Statistical chart of the proportion of inhibitory phenotypic molecules in CD4⁺CCR8⁺ Tregs in HC-PBL (n = 10), OC-PBL (n = 16) and OC-TIL (n = 12) groups. E Statistical chart of the mean fluorescence intensity (MFI) of inhibitory phenotypic molecules in CD4⁺CCR8⁺ Tregs in HC-PBL (n = 10), OC-PBL (n = 16) and OC-TIL (n = 12) groups. Data are presented as means ± SD and analysed with Student’s t-test. (*P < 0.05; **P < 0.01; ***P < 0.001)