Fig. 2

The proportion of CD4⁺Foxp3⁻CCR8⁺/CD4⁺Foxp3⁺CCR8⁺ cells, CD4⁺CCR8⁻/CD4⁺CCR8⁺ Tregs and the mean fluorescence intensity of CCR8 on HC-PBL, OC-PBL and OC-TIL groups A Representative flow cytometry analysis of CD4⁺Foxp3⁻CCR8⁺/CD4⁺Foxp3⁺CCR8⁺ cells in HC-PBL group (Foxp3 was used for gating CD4⁺CCR8⁺ cells). B Representative flow cytometry analysis of CD4⁺Foxp3⁻CCR8⁺/CD4⁺Foxp3⁺CCR8⁺ cells in OC-PBL group (Foxp3 was used for gating CD4⁺CCR8⁺ cells). C Representative flow cytometry analysis of CD4⁺Foxp3⁻CCR8⁺/CD4⁺Foxp3⁺CCR8⁺ cells in OC-TIL group (Foxp3 was used for gating CD45⁺CD3⁺CD4⁺CCR8⁺ cells). D Statistical chart of the percentage of CD4⁺Foxp3⁻CCR8⁺/CD4⁺Foxp3⁺CCR8⁺ cells in HC-PBL (n = 16), OC-PBL (n = 16) and OC-TIL (n = 16) groups. E Representative flow cytometry analysis of CD4⁺CCR8⁻/CD4⁺CCR8⁺ Tregs in HC-PBL group (CCR8 was used for gating CD4⁺Foxp3⁺ cells). F Representative flow cytometry analysis of CD4⁺CCR8⁻/CD4⁺CCR8⁺ Tregs in OC-PBL group (CCR8 was used for gating CD4⁺Foxp3⁺ cells). G Representative flow cytometry analysis of CD4⁺CCR8⁻/CD4⁺CCR8⁺ Tregs in OC-TIL group (CCR8 was used for gating CD45⁺CD3⁺CD4⁺Foxp3⁺ cells). H Statistical chart of the proportion of CD4⁺CCR8⁻/CD4⁺CCR8⁺ Tregs in HC-PBL (n = 22), OC-PBL (n = 16) and OC-TIL (n = 13) groups. I Statistical chart of the proportion of CD4⁺CCR8 ⁺ Tregs in HC-PBL (n = 22), OC-PBL (n = 16) and OC-TIL (n = 13) groups. J Statistical chart of the mean fluorescence intensity (MFI) of CCR8 on HC-PBL (n = 22), OC-PBL (n = 16) and OC-TIL (n = 13) groups. Data are presented as means ± SD and analysed with Student’s t-test. (**P < 0.01; ***P < 0.001; ****P < 0.0001)