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Table 2 PCR analysis of MSCs in P1 for IDO, CIITA, TGF-β1 and HLA-DR (flow cytometry)

From: Expression of HLA-DR by mesenchymal stromal cells in the platelet lysate era: an obsolete release criterion for MSCs?

MSC-181116a

2,3-IDO ng/µL

CIITA ng/µL

TGF-β1 ng/µL

HLA-DR (%)

MSCs-P1/PL-269

bND

52.1

ND

23.4

MSCs-P1/PL269 + IFN-γ 24 h

39.1

63.9

ND

95.0

MSCs-P1/PL269 + IFN-γ 48 h

34.5

54.6

1.7

95.0

  1. aMSCs of donor 181,116 after generation from BM-MNCs at P0 expressed high levels of HLA-DR, P0 = 31%
  2. bND = Not Detected