Expression of HLA-DR by mesenchymal stromal cells in the platelet lysate era: an obsolete release criterion for MSCs?

Table 1 Primers used for the amplification reaction

Target	Primer sequence	Product size (bp)	(References)
IDO	F 5‘-CGC TGT TGG AAA TAG CTT C-3’	234	[40]
IDO	R 5‘-CAG GAC GTC AAA GCA CTG AA-3’	234	[40]
TGF-ß1	F 5‘-CAG ATC CTC TCC AAG CTG-3’	270	[40]
TGF-ß1	R 5‘-TCG GAG CTC TGA TGT GTT-3’	270	[40]
CIITA- P/S/T	F-5‘-GAA AAG ACC CTT CCC AGAGG-3’	201	[19]
CIITA- P/S/T	R-5‘-GGG AAT CTG GTC GGT TTT CT-3’	201	[19]
CIITA-LRR	F- 5‘-GGG AAA GCT TGT GCA GAC TC-3’	199	[19]
CIITA-LRR	R- 5‘-GGG AAA GCT TGT GCA GAC TC-3’	199	[19]
GAPDH	F-5‘-CAC CAC CAT GGA GAA GGC TGG-3’	552	[33]
GAPDH	R-5‘-GAA GTC AGA GGA GAC CAC CTG-3’	552	[33]

PCR conditions used: 3 min at 95 °C/1 min at 96 °C; 3 temperatures for 10 cycles: 94 °C for 30 s, 64 °C (with a 1 °C decrease per cycle) for 30 s and 70 °C for 45 s; 3 temperatures for 28 cycles: 90 °C for 30 s, 55 °C for 30 s and 70 °C for 30 s; and hold at 60 °C for 30 min. RT‒PCR was analyzed with a capillary electrophoresis machine (2100 Bioanalyzer, Agilent, Germany)

ISSN: 1479-5876