Fig. 6
The effect of TGF-β1 on HLA-DR expression by MSCs and RT‒PCR analysis of CIITA, IDO and TGF-β1. A The effect of exogenously added TGF-β1 at repetitive doses on the expression of HLA-DR by MSCs in P0- and P1-cultured PL-145 (green circles), PL-146 (purple circles), PL-172 (orange circles) and PL-173 (gray circles) cells as measured by flow cytometry. Values represent the mean ± SD (paired t test) of 4 independent experiments with 4 different PLs. B RT‒PCR analysis of MSCs in P1 after repetitive treatment with TGF-β1 from P0 to P1. RT‒PCR of P1 MSCs generated in PL-172 cells. Lane L: loading marker, Lane 1: H2O, Lane 2 to Lane 6: MSCs untreated, control: Lane 2: GAPDH, Lane 3: IDO, Lane 4: TGF-β1, Lane 5: CIITA (upstream region), Lane 6: CIITA (downstream region), Lane 7 to Lane 11: MSCs treated with TGF-β1, Lane 7: GAPDH, Lane 8: IDO, Lane 9: TGF-β1, Lane 10: CIITA (upstream region), Lane 11: CIITA (downstream region). Lane 12: control (water instead of cDNA in PCR). C RT‒PCR products of MSCs in P1 after repetitive treatment with TGF-β1 from P0 to P1. MSCs-P1 were generated in PL-146. Designation of lines is the same as in (B). D RT‒PCR products (mRNAs) for IDO and CIITA in MSCs cultured with PL-145 (green circles), PL-146 (purple circles), PL-172 (orange circles) and PL-173 (gray circles) ± TGF-β1. E Concentration of cytokines in platelet lysates that were used for culture of MSCs in all experiments presented in this figure