Fig. 4

Cytokine levels in PLs and their impact on the expression of HLA-DR. A The levels of 11 cytokines in 10 PLs used for experiments. B MSCs from one donor were generated from BM-MNCs cultured in 10 different PLs. HLA-DR levels were measured by flow cytometry in P0 MSCs cultured in PL-145, PL-146 PL-166, PL-170, PL-171, PL-172 and PL-173, PL-175, PL-179 and PL-182 depicted with different colors. MSCs were expanded to P1 with the same PLs (except PL-175, PL-179 and PL-182), and HLA-DR expression of MSCs-P1 was assessed. Analysis of the results was performed by the Mann‒Whitney test (U test) because the samples did not show a normal distribution. C Spearman correlation analysis between the cytokine levels in PLs and expression of HLA-DR by MSCs