Fig. 2

Expression of HLA-DR in primary MSCs (P0) and P1 generated from 3 different bone marrow donors in 3 different PLs. A Generation of MSCs from BM-MNCs. B Expansion of MSCs in 3 different PLs. Number of expanded MSC-P1 from 5 × 10⁵ MSC-P0. C Comparison of HLA-DR expression by MSCs of 3 donors in P0 and the same expanded MSCs in 3 different PLs in P1 (D). Comparison of HLA-DR expression by MSCs in P0 and P1 of donor 1 (E), donor 2 (F) and donor 3 (G). The results are presented as the mean ± SD of 3 experiments. Mean values from the same donor in different PLs were compared by using a paired t test, whereas comparison of mean values between different donors was performed by an unpaired t test