Fig. 2
New bone formation within the medullary cavity of NSG mice treated with MSC, WJ. a Representative HE staining of longitudinal tibia sections six weeks after treatment. Black arrows indicate the presence of osteoblasts and asterisks indicate new bone trabeculae formed within the medullary cavity. Images in the right column are a magnification of images in the left column. b Representative images of HE, ALP, and OCN staining in longitudinal sections of tibias from MSC,WJ-treated animals illustrating the different stages of the intramembranous ossification process promoted by MSC,WJ. Black arrows indicate osteoblasts showing positive expression of ALP and asterisks indicate new bone trabeculae. Scale bar: 100 µm. NT non-treated; WT wild-type; FFly FireFly luciferase; NLuc NanoLuciferase; HE haematoxylin–eosin; ALP Alkaline Phosphatase; OCN osteocalcin