Fig. 1

Establishment of HNSCC polyculture models and culture monitoring over time. A Schematic of 2D-4-culture and 3D-4-culture: mixed cells (FaDu, HF, and THP-1 cells) were inoculated into conventional cell culture plate and SPL3D cell floater plate respectively, and single HUVEC cells were inoculated into cell culture insert. Representative bright field microscopy images of 2D-4-culture (B) and 3D-4-culture (C). Scale bar: 50 μm