Fig. 3

Treatment of HCC cells with tigecycline increases ROS levels. ELISA (A) and flow cytometry (B) based measurement of ROS levels in Huh7 and HepG2 after 48 h of treatment with 10 µM tigecycline. Bar graphs represent the mean ± SD; *p < 0.05, **p < 0.01, ***p < 0.005, ****p < 0.001; ns = no significance compared with the control group (grey bar graphs). RFU relative fluorescent units; Tige, tigecycline