Fig. 8

D594A mutant results in tumor repression upon PD-L1 blockade. WT and D594A mutant MC38 cells were inoculated subcutaneously (n = 5 mice per group). Anti-PD-L1 was administrated on day 9 and three times a week. The tumor sizes and growth curves of treated and control groups were shown as (A, B). C Analysis of the population of TILs by flow cytometry, including CD3, CD4, CD8, Macrophage and DC cells. D A schematic show that the upregulation of THBS1 promotes the secretion of CXCL9/10 and enhances CD8⁺ T cells recruitment into D594A, thereby enhancing the anti-PD-L1 response, together with the upregulation of MHC class I and PD-L1. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001