Fig. 6

THBS1 improves CXCL9/CXCL10 chemokines release in D594A mutant cells. A The mRNA levels of CXCL9, CXCL10 and CXCL11 in BRAF WT and mutant MC38 cells detected by qRT-PCR. B The concentrations of CXCL9 and CXCL10 in the supernatant of BRAF WT and mutant cells analyzed by ELISA. C The concentrations of CXCL9 and CXCL10 in the supernatant of hTHBS1 and hctrl cells detected by ELISA. D The mRNA expressions of CXCL9 and CXCL10 in hTHBS1 and hctrl cells detected by qRT-PCR. E The concentrations of CXCL9 and CXCL10 in the supernatant of siTHBS1 and sictrl cells analyzed by ELISA. F The mRNA expressions of CXCL9 and CXCL10 in siTHBS1 and sictrl cells detected by qRT-PCR. Cells were treated by 20 ng/mL IFN-γ for 48 h. Three independent replicates were performed for above experiments. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001