Fig. 5

D594A mutation reprograms transcriptome involved in cell chemotaxis and upregulates THBS1 expression via ATF3. A Analysis of differentially expressed genes (DEGs) between D594A and WT cells by RNA-seq. B Functional enrichment of DEGs by online Metascape analysis. C The potential relationship between THBS1 and CXCL10 revealed by bioinformation prediction. D The basal and induced expressions of THBS1 in BRAF WT and mutant cells detected by qRT-PCR. Cells were treated by 20 ng/mL IFN-γ for 48 h. E The protein level of THBS1 in BRAF WT and mutant cells detected by western blot. F The putative binding sites of ATF3 on THBS1 promoter predicted by jaspar website. G The ATF3 mRNA level in BRAF WT and mutant cells detected by qRT-PCR. H The transcriptional regulation of THBS1 by ATF3 examed by dual-luciferase reporter assay. EV, empty vector; NC, negative control. *P < 0.05; **P < 0.01; ***P < 0.001