Fig. 4

D594A mutation enhances CD8⁺ T cell effector function in vitro. A Analysis of the survival of CD8⁺ T cells by flow cytometry. B, C Analysis of the population expressing PD-1, TIM3, TNF-α and IFN-γ in CD8⁺ T cells by flow cytometry. CD8⁺ T cells were co-cultured with different tumor supernatants for 48 h. Three independent replicates were performed for above experiments. **P < 0.01; ***P < 0.001