Fig. 3

BRAF mutation influences the panel of TILs in transplanted tumor. BRAF WT and mutant MC38 cells were inoculated subcutaneously in homologous C57BL/6J mice (n = 5 mice per group). The tumor sizes, weights and growth curves of the four groups were shown as (A–C), respectively. D Analysis of the population of TILs by flow cytometry, including CD3, CD4, CD8, NK cells and Macrophage. E Representative immunohistochemical staining for CD8α in paraffin section of transplanted tumors (magnification 400 ×). *P < 0.05; **P < 0.01; ***P < 0.001