Fig. 2

BRAF mutation modulates the expression of immune regulatory molecules. A The effect of IFN-γ treatment on the survival of tumor cells analyzed by flow cytometry. B, C The basal and induced expressions of PD-L1 in BRAF WT and mutant MC38 cells detected by flow cytometry and qRT-PCR. D The basal and induced expressions of MHC class I in BRAF WT and mutant cells detected by flow cytometry. E–G The basal and induced expressions of Psmb8, H2-D1 and B2M in BRAF WT and mutant cells detected by qRT-PCR. Cells were treated by 20 ng/mL IFN-γ for 48 h. Three independent replicates were performed for above experiments. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001