Fig. 4

MSI-1436 ameliorates disturbances of AMPK signalling in foam cells. A Western blots from stimulation experiments with varying doses of MSI-1436 over a 3h time course B) Western blot quantification of whole protein levels and phosphorylation levels of the known PTP1B targets AMPK, JAK and STAT3 as well as total protein levels of ABCA1, SREBP1c, PTP1B and LXRa between foam cells and Thp1-derived macrophages. Quantification was performed using Image J software. B Quantification of baseline protein phosphorylation levels of AMPK, JAK2 and STAT3 between FC and M. C Quantification of baseline total protein levels of AMPK, JAK2 and STAT3 between FC and M. D Quantification of baseline total protein levels of ABCA1, LXRα, PTP1B and SREBP1c between FC and M. Data are represented as mean ± S.E.M. and were analyzed by bootstrapped t-tests