Table 2 Univariate and multivariate regression analysis of prognostic factors correlated with postoperative OS

Gender

 Female

Reference

 Male

1.10 (0.73 to 1.66)

0.640

Age

  < 60 years

Reference

  > 60 years

1.30 (0.85 to 1.99)

0.223

Gall stone

 Absent

Reference

 Present

1.36 (0.91 to 2.06)

0.137

Tumor size

 < 3 cm

Reference

 > 3 cm

1.15 (0.76 to 1.73)

0.513

TNM stage

 Low (I.II. III)

Reference

Reference

Reference

 High(IV)

6.11 (3.84 to 9.72)

 < 0.001

4.04 (2.07to7.87)

 < 0.001

4.14 (2.12 to 8.09)

 < 0.001

Surgical margin

 R0

0.16 (0.10 to 0.25)

 < 0.001

0.51 (0.28to0.92)

0.026

0.51 (0.28 to 0.92)

0.025

 R1

Reference

Reference

Reference

Adjuvant therapy

 Present

1.14 (0.74 to 1.73)

0.555

0.58 (0.36to0.92)

0.021

0.59 (0.37 to 0.94)

0.027

 Absent

Reference

Reference

Reference

Histologic differentiation

 Moderate. Well

Reference

Reference

Reference

 Poor

7.04 (4.36 to 11.36)

 < 0.001

5.06 (3.00to8.55)

 < 0.001

5.05 (3.00 to 8.53)

 < 0.001

TIM3

 Low

Reference

Reference

 High

1.57 (1.03 to 2.38)

0.036

1.16 (0.70to1.91)

0.561

PD1

 Low

Reference

Reference

 High

2.15 (1.37 to 3.36)

 < 0.001

1.54 (0.91to2.62)

0.111

GROUP

 GROUPI

Reference

Reference

 GROUPII

1.56 (0.88 to 2.77)

0.13

1.54 (0.86 to 2.76)

0.142

 GROUPIII

2.27 (1.36 to 3.77)

0.002

1.80 (1.08 to 3.02)

0.025