Fig. 4

Increased co-expression of PD1 and TIM3 is associated with elevated infiltration density of immune cell at the hepatic invasion margin (HIM). A, The donut plots show the proportion of cases with liver invasion in each subgroup. These subgroups were grouped according to PD1/TIM3 expression status. B, Representative IHC images of CD8, CD4, CD68, and Foxp3 staining within the hepatic invasion margin (Red scale bar is 50 μm). C, Bar plots show that in the PD1/TIM3 co-expression subgroup, more patients have higher counts of immune cells at the hepatic invasion margin than the tumor core. D, Representative mIHC images of PD1, TIM3, and CD8 staining on the Hepatic invasion margin (White scale bar is 400 μm; Red scale bar is 30 μm; White dotted line marks the invasive border)