Fig. 3

The correlation between the expression of PD1/TIM3 and the infiltration density of immune cells. A, Representative IHC images of CD8, CD4, CD68, and Foxp3 staining within the tumor (Red scale bar is 50 μm). B, The chord diagram shows the correlation network between immune checkpoints H score and immune cell density in GBC tissue. The band represents a positive correlation between the ICP and immune cell density, and the width indicates the magnitude of Pearson’s correlation coefficient (the P value for testing the correlation coefficient was < 0.05). C, The correlation of PD1/TIM3 co-expression status and CD8 + TIL, CD4 + TIL, CD68 + cell, Foxp3 + TIL density in GBC tissue (*P < 0.05; **P < 0.01; ***P < 0.001; ns: no significance). D, Representative mIHC images of PD1, TIM3, CD8, and Foxp3 staining within the tumor (White scale bar is 20 μm), yellow arrows indicate the colocation of PD1 and TIM3 on Foxp3 + TIL. E, The percentage of Foxp3 + TIL with different PD1/TIM3 co-expression status in GBC tissues. F, Kaplan–Meier survival curves for postoperative OS according to PD1 and TIM3 co-expression status in the high/low CD8 + TIL infiltration subgroup