Fig. 1

Expression of immune checkpoints in tumor tissues from GBC patients. A–E, Representative IHC images of PD1, TIM3, TIGIT, LAG3, and CTLA4 expression within the tumor (Red scale bar is 50 μm). F, The correlation matrix of the expression H-score of immune checkpoints, and the coefficients marked in boxes indicate the correlation between a pair of immune checkpoints. G, Upset plot visualizing the co-expression profiles of ICPs in GBC. H, Representative mIHC images of PD1 and TIM3. The nucleus is labeled with DAPI (blue), and White arrows indicate the colocation of PD1 and TIM3