Fig. 4

AAA-1 pro-inflammatory effects are TLR2-mediated. Levels of IL-6 (a), IL-8 (b) and TNF-α (c) are measured in the supernatant after cell treatment with AAA-1 alone or in combination with blocking antibodies to TLR-2, TLR-4, and CD-14. Results are expressed as median with IQR and range of six independent experiments (N = 6). Panel a) **P = 0.007, ***P = 0.001; panel b) *P = 0.038, ***P = 0.001; panel c) *P = 0.016, ***P = 0.001 by Mann–Whitney test. Levels of IL-6 (d), IL-8 (e) and TNF-α (f) are measured in the supernatant after cell treatment with AAA-1 alone or in combination with two different siRNAs targeting mSREBP1, or one siRNA used as control. Results are expressed as median with IQR and range of four independent experiments (N = 4)