Fig. 8From: Knockdown of NR3C1 inhibits the proliferation and migration of clear cell renal cell carcinoma through activating endoplasmic reticulum stress–mitophagyKnockdown of NR3C1 inhibits the proliferation and migration of ccRCC by activating ATF6. A Schematic overview for B–K. B Inhibition of ATF6 rescued the NR3C1 knockdown-mediated reduced colony formation ability of ACHN and 786-O cells, as assessed using clone formation assays. (Scale bar =100 μm). C Bar graphs represent the colony formation results in ACHN and 786-O cells. Inhibition of ATF6 rescued the NR3C1 knockdown-mediated altered proliferative capacity of ACHN (D) and 786-O (E) cells, as assessed using EdU assays. (Scale bar =100 μm). F Bar graphs represent the EdU results in ACHN and 786-O cells. Inhibition of ATF6 rescued the NR3C1 knockdown-mediated altered migration ability of ACHN (G) and 786-O (H) cells, as assessed using wound healing assays. Images were acquired at 0, 8 and 16h. (Scale bar =700 μm). I Inhibition of ATF6 rescued the NR3C1 knockdown-mediated reduced migration ability of ACHN and 786-O cells, as assessed using Transwell assays. Images were acquired at 12h. (Scale bar =100 μm). J Bar graphs show the scratch healing area in ACHN and 786-O cells at 8 and 16h. K Bar graphs represent the results of Transwell assays in ACHN and 786-O cells. The cells in the rescue group were pretreated with 20 µM ATF6i for 24h. Data presented as mean ± SD with three replicates. Ns, no significant difference; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. ATF6i, Ceapin-A7 as ATF6 inhibitorBack to article page