Fig. 4

Evaluation of TAC experiment with Gomori silver staining. A Representative images of cross sections from the inner wall of the left ventricle, B of cross sections from the inner wall of the right ventricle and C of longitudinal sections from the mid-wall of the left ventricle of sham and TAC mice, Gomori silver staining (scale = 20 µm). D Cardiomyocyte area and MinFeret of sham mice (n = 9) and TAC mice (n = 9) from three cross section images per animal of the inner wall of the left ventricle with manual correction as mean value per animal, E as violin diagram of single cardiomyocytes of sham mice (n = 7401) and TAC mice (n = 6069), Median (horizontal line), 25th and 75th centiles (dotted line), and F Standard deviation (SD) of single cardiomyocytes per animal. G Cardiomyocyte area and MinFeret of sham mice (n = 9) and TAC mice (n = 9) from three cross section images per animal of the inner wall of the left ventricle without manual correction, H from three cross section images per animal of the inner wall of the left ventricle from the second batch with manual correction and I from three cross section images per animal of the outer wall of the left ventricle with manual correction and J the inner wall of the right ventricle and with manual correction. K Cardiomyocyte area and MinFeret of sham mice (n = 9) and TAC mice (n = 9) from three longitudinal section images per animal of the mid-wall of the left ventricle with manual correction. L Cardiomyocyte nuclei area and MinFeret of sham mice (n = 9) and TAC mice (n = 9) from three cross section images per animal of the inner wall of the left ventricle with manual correction