Fig. 3

Processing of WGA+CD31+DAPI in ImageJ. Merge images are split into single channels first. The WGA channel is segmented using watershed segmentation. The user defines the tolerance and can add missing lines if necessary. The cardiomyocytes (CM) are identified by their size. Nuclei of cardiomyocytes and capillaries are identified in their appropriate channel by auto-threshold. By using the instances of the cardiomyocytes noncardiomyocyte nuclei can be excluded and the relationship of capillaries per cardiomyocyte can be evaluated. An overview image with all evaluated instances is created