Fig. 2

Processing of Gomori silver staining in ImageJ. First, segmentation is performed using watershed segmentation. The user defines the tolerance and can add missing lines if necessary. Cardiomyocytes (CM) are identified by size and color in all instances. Capillaries are identified by their position between cardiomyocytes and size. Nuclei of cardiomyocytes are identified by size and location within cardiomyocytes. The ratio of cardiomyocytes to capillaries is evaluated. An overview image with all evaluated instances is created