Fig. 1
From: Semiautomated pipeline for quantitative analysis of heart histopathology
Overview of the analysis pipeline HeartJ. Gomori silver staining (Bright-field) or immunofluorescence staining (WGA (green), DAPI (blue), CD31 (red)) images can be acquired by microscopes or scanners. Macros created for different types of images can analyze the size of cardiomyocytes, nuclei of cardiomyocytes, and capillaries. In addition, the relationship between capillaries and cardiomyocytes can be analyzed, as well as the expression of an intracardiomyocytic signal. The results are exported as an Excel file, and an overview of the analyzed image is also created, enabling manual correction of the results