Fig. 4
Candidate selection for the treatment of Lmna DCM. (a) Volcano plot showing differentially expressed genes in heart samples of Lmna DCM mice. Downregulated genes are reflected in green and upregulated genes in red. (b) Hallmark signature depicting top 10 dysregulated pathways in Lmna DCM mice as designated by GSEA, arranged by Normalised Enrichment Score (NES). (c) Western blot and quantitative analysis of phospho-Smad2, phospho-Smad3, phospho-p38, phospho-p70S6K and phospho-mTOR protein levels in mouse heart tissues of control or Lmna DCM mice, n = 5, two-tailed, unpaired T-test with Welch correction. (d) Schematic diagram of selected candidates in the cytoplasm and nucleus of cardiomyocyte as well as extracellular matrix (ECM)