Fig. 3

Increased dose of Lamin A in wildtype mice. (a) Experimental timeline showing timepoints of virus injection and echocardiogram. SR and H&E staining of paraffin heart Section 4 weeks after transduction of EGFP control or Lamin A. Quantification of myocardial fibrosis of SR sections, virus dose, 2.0E + 13 vg/kg, n = 5, two-tailed, unpaired T-test with Welch correction. For complete heart images: magnification = 4 × , scale bar = 1000 µm; for enlarged images: magnification = 20 × , scale bar = 100 µm. (b, c) Quantitative real-time PCR analyses of Nppa, Nppb, Col1a1 and Col1a2 in mice transduced with EGFP control or Lamin A, n = 5, two-tailed, unpaired T-test with Welch correction and Mann–Whitney test. (d) Western blot and quantitative analysis of phosphorylated Smad2 (p-Smad2) protein levels in mouse heart tissue of mice transduced with EGFP control or Lamin A, n = 5, two-tailed, unpaired T-test with Welch correction. (e–g) Paraffin heart sections (left) and quantifications (right) of (e) αSMA (red), (f) Iba-1 (red), (g) CD3 (red), cTnI (green) and DAPI (blue) positive cells in mice after transduction of EGFP control or Lamin A, n = 5, two-tailed, unpaired T-test with Welch correction and Mann–Whitney test, scale bar = 50 µm