Fig. 6

Identification of hDPSC. A Surface biomarkers STRO-1, CD90, and CD44 were detected to identify hDPSCs followed by flow cytometry. B The representative microscopic morphology of colony formation. The magnification is 40. C The representative crystal violet staining of colony formation. D The representative microscopic observation of sphere formation. The magnification is 200. E The growth curve of selected hDPSCs. F Representative immunofluorescence images of neural-related biomarker GFAP and Nestin on the 8-day differentiated cells from hDPSCs in neural induction medium. 1# and 2# represented two independent fields. The magnification of the left side is 200, and the right side is 400. G Representative image of Alizarin red staining. The magnification of the microscope is 200. H Representative image of Oil Red O staining. The magnification is 400