Fig. 5

Mechanism of the tumor-killing effect of YSCH-01/hDPSC. A Co-culture between tumor cell SCC152 and hDPSCs or YSCH-01/hDPSCs. The magnification is 100. B–F The cell viability of normal cell line and tumor cells under the conditioned media from YSCH-01/hDPSCs’ supernatant. Figure B is the result of the normal cell line MCF-10A. Figure C-E show the results of tumor cell line SW780, SCC152, and HCC1806. Figure F shows the results of tumor cell line U5A, which has the IFNaR2 receptor deficiency signaling. G The tumor-killing effect of YSCH-01/hDPSCs’ supernatant on SW780-EGFP cells pre-treated with YSCH-01 adenovirus neutralizing antibody (NAb). The magnification is 100. The cells with green fluorescence are the tumor cells. H The cell viability of SW780 under the conditioned medium, including NAb. The GM2 was the 48-h supernatant from the growth medium of hDPSCs and the CM2 indicated the 48-h supernatant from YSCH-01/hDPSCs