Fig. 7

Activation of the PI3K/Akt pathway is essential for the induction of HOXD9/miR-451a/PSMB8 axis-mediated ATC malignant progression. A–C Detection of HOXD9 and PSMB8 expression in the presence or absence of PSMB8 by qPCR and western blotting. D GSEA diagram was used to analyze the correlation between HOXD9 expression and PI3K/AKT signaling in GEO dataset (GSE76039). E PI3K/AKT signaling pathway proteins were measured by western blotting. F Transwell migration assays. G Cell proliferation was detected by EdU staining, scale bar = 50 μm. H, I The expression of EMT-related proteins were detected by immunofluorescence, scale bar = 50 μm. The data represent mean values ± SD, n = 3, **P < 0.01