Fig. 4

ICAM2 suppresses the GC metastasis. a–d Transwell assay investigating the effect of ICAM2 overexpression or knockdown on cell migration and invasion. e, f Results of the wound healing assay demonstrating the effect of ICAM2 overexpression or knockdown on cell migration. g Morphological changes in SGC7901 and BGC823 cells were visualized under an inverted microscope following ICAM2 overexpression or knockdown. h Western blot analysis showing the effect of ICAM2 overexpression or knockdown on the expression of EMT-related proteins. i, j Evaluation of the metastatic potential of cancer cells in vivo by injecting MGC803 (Vector and ICAM2-OE) and MKN45 (Shcon, ICAM2-sh1, and ICAM2-sh3) cells into the tail vein of nude mice. The lungs with metastatic lesions were collected and subjected to histopathological examination using H&E staining and Ki67 staining