Fig. 4

 F. nucleatum promotes the migration of CRC cells by upregulating MMP7. A, B The mRNA expression of MMP7 in CRC cells transfected with MMP7 shRNA lentivirus or control lentivirus (n = 4). C–I CRC cells were transfected with MMP7 shRNA lentivirus or control lentivirus, and then incubated with F. nucleatum or PBS. The mRNA expression (C, D) and protein expression (E, F) of MMP7 were measured by qPCR (n = 4, One-way ANOVA test) and western blot (repeated three times), respectively. The motility was detected by wound-healing assay (G, H) (n = 5, One-way ANOVA test) or transwell assay (I) (n = 6, One-way ANOVA test). Data are shown as mean ± SD, * p < 0.05, ** p < 0.01, *** p < 0.001, p < 0.001, **** p < 0.0001