Fig. 3

 F. nucleatum upregulates MMP7 in CRC cells. A, B Volcano map (A) and heat map (B), representing the differentially expressed genes between F. nucleatum-infected and PBS-treated LoVo cell by RNA-seq (n = 3 per group, logFC ≥ 2, adjusted P value < 0.05). C The expression of MMP7 among all MMPs in LoVo cells incubated with F. nucleatum or PBS was represented by heat map (n = 3 per group). D, E The mRNA expression of MMP7 after F. nucleatum, E. coli or PBS treatment in CRC cells (n = 4 per group, One-way ANOVA test). F, G The mRNA expression of MMP7 after PBS treatment or F. nucleatum infection of MOI = 100 or 500 in CRC cells (n = 4 per group, One-way ANOVA test). H The mRNA expression of MMP7 at 0 h, 12 h, and 24 h after F. nucleatum infection in HCT116 cells (n = 4 per group, One-way ANOVA test). I The protein expression of MMP7 after PBS treatment, F. nucleatum infection of MOI = 100 or 500, or E. coli infection of MOI = 100 in CRC cells (repeated three times). J The mRNA expression of MMP7 after F. nucleatum infection in NCM460 cells (n = 4 per group). Data are shown as mean ± SD, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001