Fig. 2

 F. nucleatum is overabundant in CRC and promotes the migration of CRC cells. A The relative abundance of fecal Fusobacterium spp. among the healthy volunteers (HV, n = 26), the patients with acute abdominal diseases (AAD, n = 13), the patients with colorectal precancerous lesion (CPL, n = 18), and the patients with colorectal cancer (CRC, n = 76, Kruskal-Wallis test). B The relative abundance of Fusobacterium spp. in stool (n = 76) and cancer tissue of colorectal cancer (n = 19, Mann Whitney U test). C The relative abundance of Fusobacterium spp. in paired cancer tissue and adjacent normal tissue (Wilcoxon test). D The abundance of F. nucleatum in paired cancer tissue (n = 19) and adjacent normal tissue (n = 19, Wilcoxon test). E, F the motility of LoVo and HCT116 cells was detected by wound-healing assay after incubation with PBS or F. nucleatum or E. coli (n = 6, one-way ANOVA). G the motility of LoVo was tested by transwell assay after treatment with PBS, F. nucleatum or E. coli (n = 5, one-way ANOVA). Data are shown as mean ± SD, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001