Table 3 Degenerate primers designed for the subcloning of the selected CD19-specific VHHs into the pET-26b(+) expression vector
From: Characterization of novel CD19-specific VHHs isolated from a camelid immune library by phage display
Designation | Sequence |
|---|---|
Forward-NcoI-Degenerate | 5′—CATGCCATGGCCATGSAGGTSCAGCTGCWGG—′3 |
Reverse-XhoI-Degenerate | 5′—CCGCTCGAGAAGATCTTCTTCGCTAATAAGTTTTTGTTCKGAGSWSACKGTSACC-3′ |