Table 2 Primers used for the second round of nested PCR for VHH library construction
From: Characterization of novel CD19-specific VHHs isolated from a camelid immune library by phage display
Pair | Designation | Sequence | References |
|---|---|---|---|
1st | Fr4-SfiI | 5′—ACTGGCCCAGGCGGCCGAGGTGCAGCTGSWGSAKTCKG—3′ | [73] |
Fr1-SfiI | 5′—ACTGGCCGGCCTGGCCTGAGGAGACGGTGACCWGGGTC—3′ | ||
2nd | VHH_For | 5′—GTTATTACTCGCGGCCCAGCCGGCCATGGCCGATGTGCAGCTGCAGGAGTCTGGRGGAGG—3′ | [74] |
VHH_Rev_IgG2 | 5′—GGTGATGGTGTTGGCCTCCCGGGCCGGCCGCTGGTTGTGGTTTTGGTGTCTT—3′ | ||
3rd | VHH_For | 5′—GTTATTACTCGCGGCCCAGCCGGCCATGGCCGATGTGCAGCTGCAGGAGTCTGGRGGAGG—3′ | [74] |
VHH_Rev_IgG3 | 5′—GGTGATGGTGTTGGCCTCCCGGGCCGGCCGCGGAGCTGGGGTCTTCGCTGTG-3' | ||
4th | VHH F | 5'-CTGGCCCAGGCGGCCGAGGTGCAGCTG(C/G)(A/T)G(C/G)A(G/T)TC(G/T)G-3' | [75] |
VHH R | 5'-ACTGGCCGGCCTGGCCTGAGGAGACGGTGATGACC(A/T)GGGTC-3' | ||
5th | Ryckaert et al. 1 | 5'-AAAGAGAGGCCGAAGCGGCCGTGCAGCTGGTGGAGTCTG-3' | [77] |
Ryckaert et al. 2 | 5'-TTCGAAGGCCCCACCGGCCGAGGAGACGGTGACCTGGGT-3' | ||
6th | VH1 | 5'-CATGCCATGACTCGCGGCCCAGGCGGCCATGGCCCAGGTGCAGCTGGTGCAGTCTGG-3' | [77] |
VH1b-SfiI | 5'-GCTGGATTGTTATTACTCGCGGCCCAGCCGGCCATGGCCCAGGTSMARCTGCAGSAGTCWGG-3' | [78] | |
7th | VH3 | 5'-CATGCCATGACTCGCGGCCCAGGCGGCCATGGCCGAGGTGCAGCTGGTGGAGTCTGG-3' | [77] |
VH1b-SfiI | 5′—GCTGGATTGTTATTACTCGCGGCCCAGCCGGCCATGGCCCAGGTSMARCTGCAGSAGTCWGG—3′ | [78] | |
8th | VH4 | 5′—CATGCCATGACTCGCGGCCCAGGCGGCCATGGCCCAGGTGCAGCTGCAGGAGTCGGG-3' | [77] |
VH1b-SfiI | 5′—GCTGGATTGTTATTACTCGCGGCCCAGCCGGCCATGGCCCAGGTSMARCTGCAGSAGTCWGG—3′ | [78] | |
9th | VH1 | 5′—CATGCCATGACTCGCGGCCCAGGCGGCCATGGCCCAGGTGCAGCTGGTGCAGTCTGG—3′ | [77] |
VH6b-SfiI | 5′—CGTGGATTGTTATTATCTGCGGCCCAGCCGGCCATGGCCGATGTGCAGCTGCAGGCGTCTGGRGGAGG—3′ | [78] | |
10th | VH3 | 5′—CATGCCATGACTCGCGGCCCAGGCGGCCATGGCCGAGGTGCAGCTGGTGGAGTCTGG—3′ | [77] |
VH6b-SfiI | 5′—CGTGGATTGTTATTATCTGCGGCCCAGCCGGCCATGGCCGATGTGCAGCTGCAGGCGTCTGGRGGAGG—3′ | [78] | |
11th | VH4 | 5′—CATGCCATGACTCGCGGCCCAGGCGGCCATGGCCCAGGTGCAGCTGCAGGAGTCGGG—3′ | [77] |
VH6b-SfiI | 5′—CGTGGATTGTTATTATCTGCGGCCCAGCCGGCCATGGCCGATGTGCAGCTGCAGGCGTCTGGRGGAGG—3′ | [78] |