Table 1 Primers used for the first round of nested PCR for VHH library construction
From: Characterization of novel CD19-specific VHHs isolated from a camelid immune library by phage display
Pair | Designation | Sequence | References |
|---|---|---|---|
1st | CH2-m-For-1 | 5′—CTGTTCCTCCTTTGGCTTCGTGTT—3′ | [68] |
Bq-CH2-ca2-R | 5′—GGTACGTGCTGTTGAACTGTTCC—3′ | [69] | |
2nd | VHBACKA6 | 5′—GATGTGCAGCTGCAGGCGTCTGG(A\G)GGAGG—3′ | [70] |
CH2FORTA4 | 5′—CGCCATCAAGGTACCAGTTGA—3′ | ||
3rd | CALL001 | 5′—GTCCTGCTGCTCTTCTACAAGG—3′ | [71] |
CALL002 | 5′—GGTACGTGCTGTTGAACTGTTCC—3′ | ||
4th | CH2-m-For-1 | 5′—CTGTTCCTCCTTTGGCTTCGTGTT—3′ | [68] |
VHH-m-Back-1 | 5′—TGGGTGGTCCTGGCTGCTCTT—3′ | ||
5th | AlpVh-LD | 5′—CTTGGTGGTCCTGGCTGC—3′ | [72] |
CH2-R | 5′—GGTACGTGCTGTTGAACTGTTCC—3′ | ||
6th | Bq-lead-lg-F | 5′—GTCCTGGCTGCTCTWYTACARGG—3′ | [69] |
Bq-CH2-ca2-R | 5′—GGTACGTGCTGTTGAACTGTTCC—3′ | ||
7th | SM017 | 5′—CCAGCCGGCCATGGCTCAGGTGCAGCTGGTGGAGTCTGG—3′ | [69] |
SM018 | 5′—CCAGCCGGCCATGGCTGATGTGCAGCTGGTGGAGTCTGG—3′ | ||
8th | Bq-lead-lg-F | 5′—GTCCTGGCTGCTCTWYTACARGG—3′ | [69] |
VHH-m-Back-1 | 5′—TGGGTGGTCCTGGCTGCTCTT—3′ | [68] |