Fig. 2
From: Tyrosine kinase signaling-independent MET-targeting with CAR-T cells
MET-CAR-T cells specifically kill HCC cells with MET overexpression in vitro. A HGF/MET expression level and pathway activity in MHCC97H, C3A, JHH5, and SNU398 (MET negative) cells. B MET-CAR-THS cell killing activity against MHCC97H, C3A, JHH5, and SNU398 cells in vitro. TC refers to tumor cells only. The MTS assay measures tumor cell viability. Survival (%) = (OD value of treated samples-OD value of blank)/ (OD value of TC-only samples-OD value of blank). Results were averaged from 5 independent experiment with PBMCs from healthy donors (n = 5). Triplicates were used for each experiment. Bracketed bar refers to standard deviation. C Confocal imaging of MHCC97HmCherry and MET-CAR-T cell co-culture. MET-CAR-T cells (green) and MHCC97HmCherry cells (red) were co-cultured at E:T = 10:1 ratio. After 24 h sequential z-stack images were acquired under mCherry (ex/em = 552/589–660), MitoTracker Green (ex/em = 488/497–549) and DIC. Scale bar refers to 100 μm