Fig. 1

MET-CAR-T cell production and characterization. A MET-CAR structure. The specific MET-targeting scFv domain is linked with a TCR transmembrane (TM) domain (CD28 TM or CD8α TM) using a SSR. MET-CAR.CD28ζ and MET-CAR.4-1BBζ vectors use a CD3ζ domain (CD28ζ or 4-1BBζ, respectively) as the TCR signaling module. MET-CARΔ vector is a negative control that does not have the CD3ζ domain. All vectors have a 2A sequence linked to CD19 as an expression marker for measuring T cell transduction efficacy using flow cytometry. B CD3⁺, CD4⁺, CD8⁺ and CD19⁺ expression in NT and MET-CAR-T cells as determined by flow cytometry. C Overall MET-CAR-T cell transduction efficacy determined by percentage of cells with CD19 expression (%). D Total CD3ζ expression in MET-CAR-T cells measured by Western blotting