Fig. 5

Siglec-15⁺ tumor cells or TAMs were strongly associated with the spatial distribution of Tregs and TAMs (n = 189). A Representative composite image, phenotype map, proximity distance map showing CD4⁺FoxP3⁺ Tregs within a 20um radius from the nuclear center of each S15⁻ and S15⁺ tumor cells, (B) or TAMs, (C) and CD68⁺ TAMs within a 20um radius from the nuclear center of each S15⁻ and S15⁺ tumor cells. Scale bar, 100 um. D The CD4⁺FoxP3⁺ Tregs density around S15⁺ TAMs was significantly higher than that around S15⁺ tumor cells. E S15⁺ tumor cells were closer proximity to CD4⁺FoxP3⁺ Tregs compared with S15⁻ tumor cells. F S15⁺ TAMs were spatially closer to CD4⁺FoxP3⁺ Tregs than S15⁻ TAMs, (G) and S15⁺ tumor cells. J S15⁺ tumor cells or TAMs were spatially closer to CD4⁺FoxP3⁺ Tregs than S15⁻ tumor cells, (K) and spatially closer to CD4⁺FoxP3⁺ Tregs than S15⁻ TAMs, regardless of PD-L1 expression. H S15⁺ tumor cells were surrounded by more CD68⁺ TAMs, (I) and spatially closer to CD68⁺ TAMs than S15⁻ tumor cells. L S15⁺ tumor cells were infiltrated by more CD68⁺ TAMs than S15⁻ tumor cells, (M) and were spatially closer to CD68⁺ TAMs than S15⁻ tumor cells, regardless of PD-L1 expression