Fig. 4

Siglec-15⁺ TAMs were more closely related to CD8⁺ T cells spatial distribution in PD-L1⁻ cells (n = 189). A Representative composite image, phenotype map, and proximity distance map showing CD8⁺ T cells within a 20um radius from the nuclear center of each S15⁻ and S15⁺ tumor cells or TAMs. Scale bar, 100 um. B Accurate spatial analysis over multiple distance ranges (< 20, 40, 60, or 80 μm) in terms of spatial density and distance. C There was no significant difference in the spatial density of CD8⁺ T cells, (D) and distance to CD8⁺ T cells between S15⁺ and S15⁻ tumor cells. E The density of CD8⁺ T cells around S15⁺ TAMs were significantly higher than that around both S15⁻ TAMs, (G) and S15⁺ tumor cells. F S15⁺ TAMs were spatially closer to CD8⁺ T cells than S15⁻ TAMs, (H) and S15⁺ tumor cells. I Lower infiltrating CD8⁺ T cells surrounding PD-L1⁻ + S15⁺ tumor cells than PD-L1⁻ + S15⁻ tumor cells. J PD-L1⁻ + S15⁺ TAMs were significantly closer proximity to CD8⁺ T cells than PD-L1⁻ + S15⁻ TAMs