Fig. 1
From: The application of HER2 and CD47 CAR-macrophage in ovarian cancer
The construction of HER2 and CD47 CAR-M and phagocytosis in vitro. A The expression and localization of HER2 and CD47 in the tumor tissues of patient with ovarian cancer. B The expression of HER2 and CD47 in two ovarian cancer cell lines SKOV3 and A2780. C The chimeric antigen receptor structure used in this study includes an anti-CD47/HER2 scFv segment, a hinge region and transmembrane domain from CD8, 4-1BB and CD3ζas intracellular domains, and a GFP fluorescent tag attached. D The experimental flow of constructing CAR-M and subsequent in vitro experiments. E The phagocytosis of HER2 CAR-M on HER2lo SKOV3 and HER2hi A2780 (n = 5). F The phagocytic effect of CD47 CAR-M on CD47hi SKOV3 and A2780 (n = 5). ns not significant; ** P < 0.01; *** P < 0.001