Fig. 5
From: Correlation of the tumor escape phenotype with loss of PRELP expression in melanoma
Altered expression of IFN-γ signal components, NLRC5 and TGF-β pathway molecules in PRELPhigh vs. PRELPlow/neg melanoma cells. A Increased mRNA levels of IRF1 and NLRC5 in PRELPhigh vs. PRELPlow melanoma cells were assessed by qPCR as described in “Methods” for IRF1 and NLRC5 expression. The qPCR data are expressed in bar charts relative to parental cells (set 1) and represent the mean ± SE format from three independent experiments. B A representative WB using an anti-IRF1 Ab is shown and the PRELP protein expression is marked. C The mRNA levels of IRF5, STAT1 and STAT2 were determined by qPCR as described in “Methods”. The qPCR data are shown in bar charts relative to parental cells (set 1) and represent the mean ± SE format from three independent experiments. D Altered TGF-β signaling components in PRELPhigh B16F10 cells. The expression levels of TGFB1, TRFBR1 and SMAD2 were determined by qPCR. The data are expressed in bar charts relative to parental cells (set 1) and represent the mean ± SE format from three independent experiments. E Reduced NK cell activity in PRELPhigh vs. PRELPlow Buf1088 cells. CD107a degranulation assay was performed as described in “Methods” by co-culture with NK cells from three different donors with PRELPlow vs. PRELPhigh Buf1088 cells. The mean ± SE of the CD107a degranulation of PRELPlow vs. PRELPhigh Buf1088 cells using NK cells representing total NK cell activity are shown. The statistical significance is presented as *p < 0.05; **p < 0.01; ***p < 0.001