Fig. 3

Restoration of PRELP expression in melanoma cells and its effect on growth properties. Murine and human PRELP^low melanoma cells by transfection. Restoration of PRELP expression. A PRELP expression in PRELP^high vs. PRELP^low expressing melanoma cells was determined by Western blot analysis as described in “Methods”. A representative WB using an anti-PRELP Ab is shown and the PRELP protein expression (37 kD) is marked. B Reduced proliferation of PRELP^high vs. PRELP^low expressing melanoma cells. The growth properties of PRELP^high vs. PRELP^low B16F10 and Buf1088 cells were determined as described in “Methods”. The proliferation rates of PRELP^low and PRELP^high melanoma cells were correlated to that of PRELP^low vector controls, which were set to “100”. The data are represented in bar charts as the mean of three independent experiments. C Altered migration capacity of PRELP^low vs. PRELP^high melanoma cells. The migration rate of PRELP^low vs. PRELP^high B16F10 and Buf1088 cells was determined by ATP-based fluorescence as described in “Methods” and normalized to the seeding control. The graph represents the % of migrated PRELP^low and PRELP^high melanoma cells as a mean of three independent experiments. D Altered cell cycle in PRELP^low vs. PRELP^high melanoma cells. Cell cycle distribution of PRELP^low vs. PRELP^high B16F10 and Buf1088 was assessed by flow cytometry as described in “Methods”. The data were presented as the % of cells in the different cell cycle phases as the mean of three independent experiments demonstrating a cell cycle arrest in the G0/G1 phase of PRELP^high melanoma cells. The statistical significance is presented as *p < 0.05; **p < 0.01; ***p < 0.001