Table 1 Primer sequences used for real-time PCR
Primer name | Sequence(5'-3') |
|---|---|
hMX-1 (F) | GTTTCCGAAGTGGACATCGCA |
hMX-1 (R) | CTGCACAGGTTGTTCTCAGC |
hIFITM1 (F) | CCAAGGTCCACCGTGATTAAC |
hIFITM1 (R) | ACCAGTTCAAGAAGAGGGTGTT |
hIFIT3 (F) | AAAAGCCCAACAACCCAGAAT |
hIFIT3 (R) | CGTATTGGTTATCAGGACTCAGC |
hISG15 (F) | CGCAGATCACCCAGAAGATCG |
hISG15 (R) | TTCGTCGCATTTGTCCACCA |
hIFI6 (F) | GGTCTGCGATCCTGAATGGG |
hIFI6 (R) | TCACTATCGAGATACTTGTGGGT |
hIFI44L (F) | ACAGAGCCAAATGATTCCCTATG |
hIFI44L (R) | TCGATAAACGACACACCAGTTG |
hIFIT1 (F) | AGAAGCAGGCAATCACAGAAAA |
hIFIT1 (R) | CTGAAACCGACCATAGTGGAAAT |
hP-53 (F) | CTGCCCTCAACAAGATGTTTTG |
hP-53 (R) | CTATCTGAGCAGCGCTCATGG |
hP-21 (F) | GCCTGGACTGTTTTCTCTCG |
hP-21 (R) | ATTCAGCATTGTGGGAGGAG |
hP-16(F) | GAAGGTCCCTCAGACATCCCC |
hP-16 (R) | CCCTGTAGGACCTTCGGTGAC |
hSOX2 (F) | GCCGAGTGGAAACTTTTGTCG |
hSOX2 (R) | GGCAGCGTGTACTTATCCTTCT |
hNANOG (F) | TTTGTGGGCCTGAAGAAAACT |
hNANOG (R) | AGGGCTGTCCTGAATAAGCAG |
hADIPOQ (F) | AACATGCCCATTCGCTTTACC |
hADIPOQ (R) | TAGGCAAAGTAGTACAGCCCA |
hPPAR-γ (F) | GGGATCAGCTCCGTGGATCT |
hPPAR-γ (R) | TGCACTTTGGTACTCTTGAAGTT |
hRUNX2 (F) | CCGCCTCAGTGATTTAGGGC |
hRUNX2 (R) | GGGTCTGTAATCTGACTCTGTCC |
hBGLAP (F) | CACTCCTCGCCCTATTGGC |
hBGLAP (R) | CCCTCCTGCTTGGACACAAAG |
hRUNX1 (F) | CTGCCCATCGCTTTCAAGGT |
hRUNX1 (R) | GCCGAGTAGTTTTCATCATTGCC |
hHOXB4 (F) | CGTGAGCACGGTAAACCCC |
hHOXB4 (R) | CGAGCGGATCTTGGTGTTG |
hPOU5F1 (F) | CTTGAATCCCGAATGGAAAGGG |
hPOU5F1 (R) | GTGTATATCCCAGGGTGATCCTC |
hSDF-1 (F) | CACTTTAGCTTCGGGTCAATG |
hSDF-1 (R) | ACACTCCAAACTGTGCCCTTCA |
hβ-actin (F) | CTACCTCATGAAGATCCTCACCGA |
hβ-actin (R) | TTCTCCTTAATGTCACGCACGATT |