Fig. 6

IFI6 promotes the proliferation of B-ALL cells by stimulating the SDF-1/CXCR4 axis to activate the ERK signaling pathway. A The expression levels of SDF-1 in MSCs with CON, EV and LV-IFI6 co-cultured with Nalm-6 (n = 4) /RS4;11 (n = 3) for 72 h, mean ± SD. B The expression levels of CXCR4 in Nalm-6/RS4;11 co-cultured with MSCs-CON, MSCs-EV and MSCs-LV-IFI6 for 72 h, mean ± SD, n = 4. C The expression levels of SDF-1 in MSCs with CON, EV and Si-IFI6 co-cultured with Nalm-6/RS4;11 for 72 h, mean ± SD, n = 4. D The expression levels of CXCR4 in Nalm-6/RS4;11 co-cultured with MSCs-CON, MSCs-EV and MSCs-Si-IFI6 for 72 h, mean ± SD, n = 4. E and F The expression levels of p-AKT and p-ERK in Nalm-6/RS4;11 co-cultured with MSCs-CON, MSCs-EV and MSCs-LV-IFI6 for 72 h, mean ± SD, n = 3. G and H The expression levels of p-AKT and p-ERK in Nalm-6/RS4;11 co-cultured with MSCs-CON, MSCs-EV and MSCs-Si-IFI6 for 72 h, mean ± SD, n = 3. I and J The numbers of cell proliferation of Nalm-6 and RS4;11 cells co-cultured with MSCs-CON, MSCs-EV, MSCs-LV-IFI6 and MSCs-LV-IFI6 + AMD3100 (20 μM) groups after 24 h, 48 h, 72 h and 96 h of cells incubation, mean ± SEM, n = 3, the “*” in the figure represents a significant difference between the MSCs-LV-IFI6 and MSCs-LV-IFI6 + AMD3100 groups. K and L The numbers of cell proliferation of Nalm-6 and RS4;11 cells co-cultured with MSCs-CON, MSCs-EV, MSCs-LV-IFI6 and MSCs-LV-IFI6 + PD98059 (20 μM) groups after 24 h, 48 h, 72 h and 96 h of cells incubation, mean ± SEM, n = 4, the “*” in the figure represents a significant difference between the MSCs-LV-IFI6 and MSCs-LV-IFI6 + PD98059 groups. *P < 0.05, **P < 0.01, ***P < 0.001